Fang, Wenjing, Chen, Jin, Huang, Xiwei, Sun, Lingling
Accepted: 2025-01-13
With the advancement of research on single-cell heterogeneity, cellular electrophysical properties have become a crucial focus in disease diagnosis and precision medicine. Microfluidic bioelectrical impedance detection chips measure the impedance changes of cells in an electric field with high precision, enabling the label-free acquisition of cellular electrophysical characteristics such as cell size, membrane capacitance, and cytoplasmic conductivity. This significantly enhances the ability to detect cellular heterogeneity. Compared to traditional methods, microfluidic bioelectrical impedance detection chip technology offers advantages such as high sensitivity, simplicity of operation, and non-destructive detection, demonstrating broad application prospects in early disease diagnosis, drug screening, and personalized treatment. This review first elaborates on the basic principles and system design of the technology, then analyzes the progress in optimizing microfluidic channels and electrode configurations, and discusses its applications in cell classification, drug evaluation, and other fields. Finally, the review examines current technical challenges and future development trends, highlighting its potential for widespread application in precision medicine and early disease diagnosis.